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Establishment of mouse RILF model and analysis of <t>transcriptome</t> <t>microarray</t> data analysis. (A) HE and masson staining of lung tissue collected at 20 weeks after IR (control, 17 Gy). Lungs treated to 17 Gy dose irradiation develop foci of fibrosis. Collagen: blue; cytoplasm/epithelia: pink. Scale bar = 20 μm. (B) The PCA plot of the distribution status of the two groups (C) Volcano plots displaying significantly differentially expressed genes. |log2FC| ≥ 0.5 and P < 0.05. (D) Heatmap displaying the expressions of the DEGs. Blue corresponds to lower gene expression and red to higher gene expression. (E) Bar plot of enriched KEGG pathways/GO terms showing DEGs.
Transcriptome Microarray Mouse Sentrix 6 V2 Whole Genome Bead Chip, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Establishment of mouse RILF model and analysis of <t>transcriptome</t> <t>microarray</t> data analysis. (A) HE and masson staining of lung tissue collected at 20 weeks after IR (control, 17 Gy). Lungs treated to 17 Gy dose irradiation develop foci of fibrosis. Collagen: blue; cytoplasm/epithelia: pink. Scale bar = 20 μm. (B) The PCA plot of the distribution status of the two groups (C) Volcano plots displaying significantly differentially expressed genes. |log2FC| ≥ 0.5 and P < 0.05. (D) Heatmap displaying the expressions of the DEGs. Blue corresponds to lower gene expression and red to higher gene expression. (E) Bar plot of enriched KEGG pathways/GO terms showing DEGs.
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Establishment of mouse RILF model and analysis of <t>transcriptome</t> <t>microarray</t> data analysis. (A) HE and masson staining of lung tissue collected at 20 weeks after IR (control, 17 Gy). Lungs treated to 17 Gy dose irradiation develop foci of fibrosis. Collagen: blue; cytoplasm/epithelia: pink. Scale bar = 20 μm. (B) The PCA plot of the distribution status of the two groups (C) Volcano plots displaying significantly differentially expressed genes. |log2FC| ≥ 0.5 and P < 0.05. (D) Heatmap displaying the expressions of the DEGs. Blue corresponds to lower gene expression and red to higher gene expression. (E) Bar plot of enriched KEGG pathways/GO terms showing DEGs.
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Establishment of mouse RILF model and analysis of <t>transcriptome</t> <t>microarray</t> data analysis. (A) HE and masson staining of lung tissue collected at 20 weeks after IR (control, 17 Gy). Lungs treated to 17 Gy dose irradiation develop foci of fibrosis. Collagen: blue; cytoplasm/epithelia: pink. Scale bar = 20 μm. (B) The PCA plot of the distribution status of the two groups (C) Volcano plots displaying significantly differentially expressed genes. |log2FC| ≥ 0.5 and P < 0.05. (D) Heatmap displaying the expressions of the DEGs. Blue corresponds to lower gene expression and red to higher gene expression. (E) Bar plot of enriched KEGG pathways/GO terms showing DEGs.
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Establishment of mouse RILF model and analysis of <t>transcriptome</t> <t>microarray</t> data analysis. (A) HE and masson staining of lung tissue collected at 20 weeks after IR (control, 17 Gy). Lungs treated to 17 Gy dose irradiation develop foci of fibrosis. Collagen: blue; cytoplasm/epithelia: pink. Scale bar = 20 μm. (B) The PCA plot of the distribution status of the two groups (C) Volcano plots displaying significantly differentially expressed genes. |log2FC| ≥ 0.5 and P < 0.05. (D) Heatmap displaying the expressions of the DEGs. Blue corresponds to lower gene expression and red to higher gene expression. (E) Bar plot of enriched KEGG pathways/GO terms showing DEGs.
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Establishment of mouse RILF model and analysis of <t>transcriptome</t> <t>microarray</t> data analysis. (A) HE and masson staining of lung tissue collected at 20 weeks after IR (control, 17 Gy). Lungs treated to 17 Gy dose irradiation develop foci of fibrosis. Collagen: blue; cytoplasm/epithelia: pink. Scale bar = 20 μm. (B) The PCA plot of the distribution status of the two groups (C) Volcano plots displaying significantly differentially expressed genes. |log2FC| ≥ 0.5 and P < 0.05. (D) Heatmap displaying the expressions of the DEGs. Blue corresponds to lower gene expression and red to higher gene expression. (E) Bar plot of enriched KEGG pathways/GO terms showing DEGs.
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Establishment of mouse RILF model and analysis of <t>transcriptome</t> <t>microarray</t> data analysis. (A) HE and masson staining of lung tissue collected at 20 weeks after IR (control, 17 Gy). Lungs treated to 17 Gy dose irradiation develop foci of fibrosis. Collagen: blue; cytoplasm/epithelia: pink. Scale bar = 20 μm. (B) The PCA plot of the distribution status of the two groups (C) Volcano plots displaying significantly differentially expressed genes. |log2FC| ≥ 0.5 and P < 0.05. (D) Heatmap displaying the expressions of the DEGs. Blue corresponds to lower gene expression and red to higher gene expression. (E) Bar plot of enriched KEGG pathways/GO terms showing DEGs.
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Establishment of mouse RILF model and analysis of <t>transcriptome</t> <t>microarray</t> data analysis. (A) HE and masson staining of lung tissue collected at 20 weeks after IR (control, 17 Gy). Lungs treated to 17 Gy dose irradiation develop foci of fibrosis. Collagen: blue; cytoplasm/epithelia: pink. Scale bar = 20 μm. (B) The PCA plot of the distribution status of the two groups (C) Volcano plots displaying significantly differentially expressed genes. |log2FC| ≥ 0.5 and P < 0.05. (D) Heatmap displaying the expressions of the DEGs. Blue corresponds to lower gene expression and red to higher gene expression. (E) Bar plot of enriched KEGG pathways/GO terms showing DEGs.
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Establishment of mouse RILF model and analysis of transcriptome microarray data analysis. (A) HE and masson staining of lung tissue collected at 20 weeks after IR (control, 17 Gy). Lungs treated to 17 Gy dose irradiation develop foci of fibrosis. Collagen: blue; cytoplasm/epithelia: pink. Scale bar = 20 μm. (B) The PCA plot of the distribution status of the two groups (C) Volcano plots displaying significantly differentially expressed genes. |log2FC| ≥ 0.5 and P < 0.05. (D) Heatmap displaying the expressions of the DEGs. Blue corresponds to lower gene expression and red to higher gene expression. (E) Bar plot of enriched KEGG pathways/GO terms showing DEGs.

Journal: Dose-Response

Article Title: Ferroptosis-Associated Extracellular Matrix Remodeling in Radiation-Induced Lung Fibrosis Progression

doi: 10.1177/15593258241289829

Figure Lengend Snippet: Establishment of mouse RILF model and analysis of transcriptome microarray data analysis. (A) HE and masson staining of lung tissue collected at 20 weeks after IR (control, 17 Gy). Lungs treated to 17 Gy dose irradiation develop foci of fibrosis. Collagen: blue; cytoplasm/epithelia: pink. Scale bar = 20 μm. (B) The PCA plot of the distribution status of the two groups (C) Volcano plots displaying significantly differentially expressed genes. |log2FC| ≥ 0.5 and P < 0.05. (D) Heatmap displaying the expressions of the DEGs. Blue corresponds to lower gene expression and red to higher gene expression. (E) Bar plot of enriched KEGG pathways/GO terms showing DEGs.

Article Snippet: Gene expression was detected using a transcriptome microarray (Mouse Sentrix-6 V2 Whole Genome Bead Chip, BD-201-0202; Illumina, Inc., San Diego, CA, USA).

Techniques: Microarray, Staining, Control, Irradiation, Gene Expression